ssr_primer
Primer Details for TRINITY_DN14261_c3_g1_i1 |
|---|
| (A)29: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTGCACCCAAGGTATGCA | TTGGCGATGCTCAACTCCAT | Start: 1285 |
| Tm | 59.961 | 60.035 | End: 1398 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | ATGGAGTTGAGCATCGCCAA | CGTCTCTCGCCTCCATCATC | Start: 1379 |
| Tm | 60.035 | 60.04 | End: 1577 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | CACTCGCACTCTCCTCCATC | CCTCTGGCGTCCATATGTCC | Start: 4424 |
| Tm | 59.898 | 59.966 | End: 4630 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CACTCGCACTCTCCTCCATC | CCCGATCGGATTCCCAGAAG | Start: 4424 |
| Tm | 59.898 | 59.966 | End: 4658 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TTGACATCACCGGCACTACC | GATGGAGGAGAGTGCGAGTG | Start: 4342 |
| Tm | 60.037 | 59.898 | End: 4443 |
| Size | 20 | 20 | 102 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTGCACCCAAGGTATGCA | TTGGCGATGCTCAACTCCAT | Start: 1285 |
| Tm | 59.961 | 60.035 | End: 1398 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | ATGGAGTTGAGCATCGCCAA | CGTCTCTCGCCTCCATCATC | Start: 1379 |
| Tm | 60.035 | 60.04 | End: 1577 |
| Size | 20 | 20 | 199 |
| Primer 3 details |
|---|
| Sequence | CACTCGCACTCTCCTCCATC | CCTCTGGCGTCCATATGTCC | Start: 4424 |
| Tm | 59.898 | 59.966 | End: 4630 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CACTCGCACTCTCCTCCATC | CCCGATCGGATTCCCAGAAG | Start: 4424 |
| Tm | 59.898 | 59.966 | End: 4658 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | TTGACATCACCGGCACTACC | GATGGAGGAGAGTGCGAGTG | Start: 4342 |
| Tm | 60.037 | 59.898 | End: 4443 |
| Size | 20 | 20 | 102 |
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