ssr_primer
Primer Details for TRINITY_DN14260_c2_g1_i1 |
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| (CTC)6tgattccgttgttgattcttcctcttcgttttcatttccgctctcgttgttgttttcgttttgttttttttcttcttctgcggcttcc(GCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGATTCGAACTGGCGCTG | ATCCACTCGCCGTCAAATGT | Start: 723 |
| Tm | 59.973 | 60.036 | End: 849 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | CATGATTCGAACTGGCGCTG | CCTGTTCACAACAAGCGCTC | Start: 723 |
| Tm | 59.973 | 60.041 | End: 965 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CGGCTCACCATCAACTCCTT | GCATGAGACGGACAGGGAAA | Start: 142 |
| Tm | 60.036 | 60.036 | End: 369 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
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| Sequence | ACATTTGACGGCGAGTGGAT | CATGGGTCTCAACGGTTCCA | Start: 830 |
| Tm | 60.036 | 59.964 | End: 1003 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | AACGCAGAGGAATCGCTTGA | CATGGGTCTCAACGGTTCCA | Start: 784 |
| Tm | 60.037 | 59.964 | End: 1003 |
| Size | 20 | 20 | 220 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGATTCGAACTGGCGCTG | ATCCACTCGCCGTCAAATGT | Start: 723 |
| Tm | 59.973 | 60.036 | End: 849 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CATGATTCGAACTGGCGCTG | CCTGTTCACAACAAGCGCTC | Start: 723 |
| Tm | 59.973 | 60.041 | End: 965 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CGGCTCACCATCAACTCCTT | GCATGAGACGGACAGGGAAA | Start: 142 |
| Tm | 60.036 | 60.036 | End: 369 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | ACATTTGACGGCGAGTGGAT | CATGGGTCTCAACGGTTCCA | Start: 830 |
| Tm | 60.036 | 59.964 | End: 1003 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | AACGCAGAGGAATCGCTTGA | CATGGGTCTCAACGGTTCCA | Start: 784 |
| Tm | 60.037 | 59.964 | End: 1003 |
| Size | 20 | 20 | 220 |
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