ssr_primer
Primer Details for TRINITY_DN14259_c1_g1_i4 |
|---|
| (TCT)5tcatcttcttcttcttcatcttcttcttccaattgaatccatggaaattttgcacaaacacagttggtttccttttctgagttgaatttct(TC)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACTGGCACTCCTTTGACG | GCATGGTACCGGCAGACATA | Start: 1329 |
| Tm | 59.966 | 59.894 | End: 1573 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AGCCTTCACCTCGAATGCAA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 465 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AAGCCTTCACCTCGAATGCA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 466 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTGCTCGCTTTGCTTTGCTT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3352 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTTGCTCGCTTTGCTTTGCT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3353 |
| Size | 20 | 20 | 101 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACTGGCACTCCTTTGACG | GCATGGTACCGGCAGACATA | Start: 1329 |
| Tm | 59.966 | 59.894 | End: 1573 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AGCCTTCACCTCGAATGCAA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 465 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AAGCCTTCACCTCGAATGCA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 466 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTGCTCGCTTTGCTTTGCTT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3352 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTTGCTCGCTTTGCTTTGCT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3353 |
| Size | 20 | 20 | 101 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACTGGCACTCCTTTGACG | GCATGGTACCGGCAGACATA | Start: 1329 |
| Tm | 59.966 | 59.894 | End: 1573 |
| Size | 20 | 20 | 245 |
| Primer 2 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AGCCTTCACCTCGAATGCAA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 465 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | TCCAGGCATTTGGATCCGAC | AAGCCTTCACCTCGAATGCA | Start: 337 |
| Tm | 60.107 | 59.963 | End: 466 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTGCTCGCTTTGCTTTGCTT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3352 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TCTTTTGGCTTGGGCTCCAA | TTTGCTCGCTTTGCTTTGCT | Start: 3253 |
| Tm | 60.106 | 59.897 | End: 3353 |
| Size | 20 | 20 | 101 |
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