ssr_primer
Primer Details for TRINITY_DN14256_c3_g1_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTTTTGGTCAAGAAGGCG | CGGTCGAGATCTGTCGTCAG | Start: 485 |
| Tm | 60.04 | 59.973 | End: 714 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
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| Sequence | CGGTTTTGGTCAAGAAGGCG | AGCACCCATCAACACCGAAT | Start: 485 |
| Tm | 60.04 | 59.962 | End: 682 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | CACCTCTCCGCTCTTGGAAG | CGGTCGAGATCTGTCGTCAG | Start: 591 |
| Tm | 60.108 | 59.973 | End: 714 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
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| Sequence | ATTCGGTGTTGATGGGTGCT | AGATGTCATGCTCGTCGTCC | Start: 663 |
| Tm | 59.962 | 59.899 | End: 781 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
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| Sequence | CGGTTTTGGTCAAGAAGGCG | TCTCGGTCGAGATCTGTCGT | Start: 485 |
| Tm | 60.04 | 60.108 | End: 717 |
| Size | 20 | 20 | 233 |
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