ssr_primer
Primer Details for TRINITY_DN14256_c2_g1_i1 |
|---|
| (CT)9cactgactacagtttggattgagaaggaatagaaaaaaaccaatcccatcaaccagc(A)17cacttagttgttctaccttttatagaaaaccccaaaccaatatcctttcaacttcacaaatctacaacc(A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGGACTGCCGAACCCTC | ATCTCACTGCTCTAGCCCCA | Start: 833 |
| Tm | 59.895 | 60.032 | End: 984 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | TCTCAACAATCATCGCCCCC | ATCTCACTGCTCTAGCCCCA | Start: 713 |
| Tm | 60.107 | 60.032 | End: 984 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | ATCAAAAGCGTCGCGATTGG | GAGGGTTCGGCAGTCCATAG | Start: 676 |
| Tm | 59.903 | 59.895 | End: 852 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | CTTCTCCAGTGGTAGCAGCC | CCAATCGCGACGCTTTTGAT | Start: 572 |
| Tm | 60.108 | 59.903 | End: 695 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CCTCTCACGTTGTAGCGGAG | ATCTCACTGCTCTAGCCCCA | Start: 740 |
| Tm | 60.179 | 60.032 | End: 984 |
| Size | 20 | 20 | 245 |
(T)10caaaaaaaaacctcttcttgtttggttacaatcccaatatcatacaggcctataagaagaaggagaaaaaaaattaaaaggg(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGGACTGCCGAACCCTC | ATCTCACTGCTCTAGCCCCA | Start: 833 |
| Tm | 59.895 | 60.032 | End: 984 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | TCTCAACAATCATCGCCCCC | ATCTCACTGCTCTAGCCCCA | Start: 713 |
| Tm | 60.107 | 60.032 | End: 984 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | ATCAAAAGCGTCGCGATTGG | GAGGGTTCGGCAGTCCATAG | Start: 676 |
| Tm | 59.903 | 59.895 | End: 852 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | CTTCTCCAGTGGTAGCAGCC | CCAATCGCGACGCTTTTGAT | Start: 572 |
| Tm | 60.108 | 59.903 | End: 695 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CCTCTCACGTTGTAGCGGAG | ATCTCACTGCTCTAGCCCCA | Start: 740 |
| Tm | 60.179 | 60.032 | End: 984 |
| Size | 20 | 20 | 245 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTATGGACTGCCGAACCCTC | ATCTCACTGCTCTAGCCCCA | Start: 833 |
| Tm | 59.895 | 60.032 | End: 984 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | TCTCAACAATCATCGCCCCC | ATCTCACTGCTCTAGCCCCA | Start: 713 |
| Tm | 60.107 | 60.032 | End: 984 |
| Size | 20 | 20 | 272 |
| Primer 3 details |
|---|
| Sequence | ATCAAAAGCGTCGCGATTGG | GAGGGTTCGGCAGTCCATAG | Start: 676 |
| Tm | 59.903 | 59.895 | End: 852 |
| Size | 20 | 20 | 177 |
| Primer 4 details |
|---|
| Sequence | CTTCTCCAGTGGTAGCAGCC | CCAATCGCGACGCTTTTGAT | Start: 572 |
| Tm | 60.108 | 59.903 | End: 695 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CCTCTCACGTTGTAGCGGAG | ATCTCACTGCTCTAGCCCCA | Start: 740 |
| Tm | 60.179 | 60.032 | End: 984 |
| Size | 20 | 20 | 245 |
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