Primer Details for TRINITY_DN14254_c8_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAATGAACCAAATGAAGGAAAA | GGGCGTGGAATTTGTGCTTT | Start: 76 |
| Tm | 57.254 | 59.967 | End: 255 |
| Size | 24 | 20 | 180 |
| Primer 2 details | |||
| Sequence | AAGGAATGAACCAAATGAAGGAAA | GGGCGTGGAATTTGTGCTTT | Start: 75 |
| Tm | 57.254 | 59.967 | End: 255 |
| Size | 24 | 20 | 181 |
| Primer 3 details | |||
| Sequence | TTGAGGTTAGCTTTCCGATAATTGAC | AGAATTCGTTTTGGGCGTGG | Start: 168 |
| Tm | 59.685 | 59.404 | End: 267 |
| Size | 26 | 20 | 100 |
| Primer 4 details | |||
| Sequence | AGGAATGAACCAAATGAAGGAAAA | TTTGGGCGTGGAATTTGTGC | Start: 76 |
| Tm | 57.254 | 60.25 | End: 258 |
| Size | 24 | 20 | 183 |
| Primer 5 details | |||
| Sequence | AAGGAATGAACCAAATGAAGGAAA | TTTGGGCGTGGAATTTGTGC | Start: 75 |
| Tm | 57.254 | 60.25 | End: 258 |
| Size | 24 | 20 | 184 |
