ssr_primer
Primer Details for TRINITY_DN14254_c5_g5_i1 |
|---|
| (AC)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAGGTGTGGACTGGCAACC | ATTTTGTAGGGTCAGGCGGG | Start: 133 |
| Tm | 59.89 | 60.034 | End: 289 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | CAGTCGTGTACATGGTGGCT | CATCAAAAGCGGCAGACCAC | Start: 2266 |
| Tm | 60.037 | 60.11 | End: 2526 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TCAGTCGTGTACATGGTGGC | CATCAAAAGCGGCAGACCAC | Start: 2265 |
| Tm | 60.038 | 60.11 | End: 2526 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TGTGTGTGTTCGGTTCAGCT | TCCACGGTGTCCATCTACCT | Start: 220 |
| Tm | 60.108 | 59.959 | End: 345 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CAGTCGTGTACATGGTGGCT | ACCACCAATCACCATGTGCT | Start: 2266 |
| Tm | 60.037 | 59.887 | End: 2511 |
| Size | 20 | 20 | 246 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAAGGTGTGGACTGGCAACC | ATTTTGTAGGGTCAGGCGGG | Start: 133 |
| Tm | 59.89 | 60.034 | End: 289 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | CAGTCGTGTACATGGTGGCT | CATCAAAAGCGGCAGACCAC | Start: 2266 |
| Tm | 60.037 | 60.11 | End: 2526 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TCAGTCGTGTACATGGTGGC | CATCAAAAGCGGCAGACCAC | Start: 2265 |
| Tm | 60.038 | 60.11 | End: 2526 |
| Size | 20 | 20 | 262 |
| Primer 4 details |
|---|
| Sequence | TGTGTGTGTTCGGTTCAGCT | TCCACGGTGTCCATCTACCT | Start: 220 |
| Tm | 60.108 | 59.959 | End: 345 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | CAGTCGTGTACATGGTGGCT | ACCACCAATCACCATGTGCT | Start: 2266 |
| Tm | 60.037 | 59.887 | End: 2511 |
| Size | 20 | 20 | 246 |
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