ssr_primer
Primer Details for TRINITY_DN14254_c5_g4_i2 |
|---|
| (TTC)5attcctctgtaactccatatctagaatact(A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTCGGTTCACAGAAAGA | GCTTGGCTGCTTCGTATTCG | Start: 2514 |
| Tm | 59.967 | 59.973 | End: 2690 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAGTTAGCCAGCCAGCCATT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2872 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAAGTTAGCCAGCCAGCCAT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2873 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | CAGCTGCTGCAAGTCACAAG | Start: 2671 |
| Tm | 59.973 | 60.04 | End: 2784 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | CCGGTCGGTTCACAGAAAGA | TCGAAGAAACGACCGTGGAG | Start: 2514 |
| Tm | 59.967 | 60.04 | End: 2673 |
| Size | 20 | 20 | 160 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTCGGTTCACAGAAAGA | GCTTGGCTGCTTCGTATTCG | Start: 2514 |
| Tm | 59.967 | 59.973 | End: 2690 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAGTTAGCCAGCCAGCCATT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2872 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAAGTTAGCCAGCCAGCCAT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2873 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | CAGCTGCTGCAAGTCACAAG | Start: 2671 |
| Tm | 59.973 | 60.04 | End: 2784 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | CCGGTCGGTTCACAGAAAGA | TCGAAGAAACGACCGTGGAG | Start: 2514 |
| Tm | 59.967 | 60.04 | End: 2673 |
| Size | 20 | 20 | 160 |
(TGC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTCGGTTCACAGAAAGA | GCTTGGCTGCTTCGTATTCG | Start: 2514 |
| Tm | 59.967 | 59.973 | End: 2690 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAGTTAGCCAGCCAGCCATT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2872 |
| Size | 20 | 20 | 202 |
| Primer 3 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | AAAGTTAGCCAGCCAGCCAT | Start: 2671 |
| Tm | 59.973 | 59.96 | End: 2873 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | CGAATACGAAGCAGCCAAGC | CAGCTGCTGCAAGTCACAAG | Start: 2671 |
| Tm | 59.973 | 60.04 | End: 2784 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | CCGGTCGGTTCACAGAAAGA | TCGAAGAAACGACCGTGGAG | Start: 2514 |
| Tm | 59.967 | 60.04 | End: 2673 |
| Size | 20 | 20 | 160 |
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