Primer Details for TRINITY_DN14249_c1_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCAATGATCGTTCGCA | TCCCTGTAGTTTACTTTGATTCAAGT | Start: 124 |
| Tm | 58.917 | 58.485 | End: 232 |
| Size | 20 | 26 | 109 |
| Primer 2 details |
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| Sequence | TGTTCCCAATGATCGTTCGC | TCCCTGTAGTTTACTTTGATTCAAGT | Start: 123 |
| Tm | 58.917 | 58.485 | End: 232 |
| Size | 20 | 26 | 110 |
| Primer 3 details |
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| Sequence | GTTCCCAATGATCGTTCGCA | CCCTGTAGTTTACTTTGATTCAAGT | Start: 124 |
| Tm | 58.917 | 57.254 | End: 231 |
| Size | 20 | 25 | 108 |
| Primer 4 details |
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| Sequence | TGTTCCCAATGATCGTTCGC | CCCTGTAGTTTACTTTGATTCAAGT | Start: 123 |
| Tm | 58.917 | 57.254 | End: 231 |
| Size | 20 | 25 | 109 |
| Primer 5 details |
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| Sequence | GTTCCCAATGATCGTTCGCAA | TCCCTGTAGTTTACTTTGATTCAAGT | Start: 124 |
| Tm | 59.533 | 58.485 | End: 232 |
| Size | 21 | 26 | 109 |
