ssr_primer
Primer Details for TRINITY_DN14245_c1_g2_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGTTGCCGCTTCTGCTTC | AGCTACACCACACCCTTTGG | Start: 403 |
| Tm | 59.969 | 59.89 | End: 678 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | CCCAGCGTCAATTTTGGAGC | CCCATTTCCAGGAAGCGCTA | Start: 136 |
| Tm | 60.109 | 60.107 | End: 289 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | GCGTCAATTTTGGAGCTCCC | CCCATTTCCAGGAAGCGCTA | Start: 140 |
| Tm | 59.829 | 60.107 | End: 289 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | TAGCGCTTCCTGGAAATGGG | AGCCACCAAGAAAACTCCCC | Start: 270 |
| Tm | 60.107 | 60.179 | End: 401 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | CCCAGCGTCAATTTTGGAGC | AGCCACCAAGAAAACTCCCC | Start: 136 |
| Tm | 60.109 | 60.179 | End: 401 |
| Size | 20 | 20 | 266 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGTTGCCGCTTCTGCTTC | AGCTACACCACACCCTTTGG | Start: 403 |
| Tm | 59.969 | 59.89 | End: 678 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | CCCAGCGTCAATTTTGGAGC | CCCATTTCCAGGAAGCGCTA | Start: 136 |
| Tm | 60.109 | 60.107 | End: 289 |
| Size | 20 | 20 | 154 |
| Primer 3 details |
|---|
| Sequence | GCGTCAATTTTGGAGCTCCC | CCCATTTCCAGGAAGCGCTA | Start: 140 |
| Tm | 59.829 | 60.107 | End: 289 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | TAGCGCTTCCTGGAAATGGG | AGCCACCAAGAAAACTCCCC | Start: 270 |
| Tm | 60.107 | 60.179 | End: 401 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | CCCAGCGTCAATTTTGGAGC | AGCCACCAAGAAAACTCCCC | Start: 136 |
| Tm | 60.109 | 60.179 | End: 401 |
| Size | 20 | 20 | 266 |
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