ssr_primer
Primer Details for TRINITY_DN14236_c2_g6_i1 |
|---|
| (TTC)8ttattaatttacctccattatcttttcttttttaaccactaat(TAA)5tatataatcccgtaataattaaattgaattcaatttaaagatttaaaaagct(A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCGTCGGAAACCCAAC | AGTTCCAACCAACCAACCCA | Start: 71 |
| Tm | 60.037 | 59.663 | End: 319 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | GAATCTCCGTCGGAAACCCA | AGTTCCAACCAACCAACCCA | Start: 69 |
| Tm | 59.752 | 59.663 | End: 319 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | GGCCCCTCTGCTTCATAGTT | Start: 71 |
| Tm | 60.037 | 59.451 | End: 349 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | TGGCCCCTCTGCTTCATAGT | Start: 71 |
| Tm | 60.037 | 60.622 | End: 350 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | TCCAACCAACCAACCCAGAA | Start: 71 |
| Tm | 60.037 | 59.368 | End: 316 |
| Size | 20 | 20 | 246 |
(CTT)9tcccttttccttaacttcaatctcttttttcgatctctctgttct(TCA)5tttttttcttctttttcttcttcttcaaacttcccacttag(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCGTCGGAAACCCAAC | AGTTCCAACCAACCAACCCA | Start: 71 |
| Tm | 60.037 | 59.663 | End: 319 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
|---|
| Sequence | GAATCTCCGTCGGAAACCCA | AGTTCCAACCAACCAACCCA | Start: 69 |
| Tm | 59.752 | 59.663 | End: 319 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | GGCCCCTCTGCTTCATAGTT | Start: 71 |
| Tm | 60.037 | 59.451 | End: 349 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | TGGCCCCTCTGCTTCATAGT | Start: 71 |
| Tm | 60.037 | 60.622 | End: 350 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | ATCTCCGTCGGAAACCCAAC | TCCAACCAACCAACCCAGAA | Start: 71 |
| Tm | 60.037 | 59.368 | End: 316 |
| Size | 20 | 20 | 246 |
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