ssr_primer
Primer Details for TRINITY_DN14234_c2_g4_i1 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCACAATCCACACCGTCC | GGTCTCAGAGGTGATGGTGC | Start: 3887 |
| Tm | 60.035 | 60.108 | End: 4122 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | GTCCCAATTGCAGCAGTTGG | GGTCTCAGAGGTGATGGTGC | Start: 3903 |
| Tm | 60.038 | 60.108 | End: 4122 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | AGACACCAAATCCCTGCAGG | ACAGCCAGACATTGACTGCA | Start: 1152 |
| Tm | 59.961 | 59.891 | End: 1279 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | GCCGAGCACATTTCCATGAC | AGCGACCTTTTATGAGCCCC | Start: 328 |
| Tm | 59.901 | 60.107 | End: 543 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | CAAGCCAAATCCACCACAGC | TTTGCCTTACAGTCGGTGCA | Start: 1758 |
| Tm | 60.038 | 60.179 | End: 2026 |
| Size | 20 | 20 | 269 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCACAATCCACACCGTCC | GGTCTCAGAGGTGATGGTGC | Start: 3887 |
| Tm | 60.035 | 60.108 | End: 4122 |
| Size | 20 | 20 | 236 |
| Primer 2 details |
|---|
| Sequence | GTCCCAATTGCAGCAGTTGG | GGTCTCAGAGGTGATGGTGC | Start: 3903 |
| Tm | 60.038 | 60.108 | End: 4122 |
| Size | 20 | 20 | 220 |
| Primer 3 details |
|---|
| Sequence | AGACACCAAATCCCTGCAGG | ACAGCCAGACATTGACTGCA | Start: 1152 |
| Tm | 59.961 | 59.891 | End: 1279 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | GCCGAGCACATTTCCATGAC | AGCGACCTTTTATGAGCCCC | Start: 328 |
| Tm | 59.901 | 60.107 | End: 543 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | CAAGCCAAATCCACCACAGC | TTTGCCTTACAGTCGGTGCA | Start: 1758 |
| Tm | 60.038 | 60.179 | End: 2026 |
| Size | 20 | 20 | 269 |
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