ssr_primer
Primer Details for TRINITY_DN14223_c1_g1_i1 |
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| (T)11ccttagcataaacaaaccaaacttctatggaagaaaatg(A)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAAGGCCATTCCCGGTT | GGGAGGAGGTTTGAGGCTTC | Start: 1513 |
| Tm | 59.886 | 60.035 | End: 1674 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | AAGAAAAGGCCATTCCCGGT | GGGAGGAGGTTTGAGGCTTC | Start: 1512 |
| Tm | 59.886 | 60.035 | End: 1674 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
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| Sequence | GTTCTCGGATAGGCACCCAG | ACCGGGAATGGCCTTTTCTT | Start: 1327 |
| Tm | 59.895 | 59.886 | End: 1531 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
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| Sequence | GTTCTCGGATAGGCACCCAG | AACCGGGAATGGCCTTTTCT | Start: 1327 |
| Tm | 59.895 | 59.886 | End: 1532 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
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| Sequence | ACTCCACAGGCCACAAACAA | CGAAGCTTCTCTCCACGTGA | Start: 619 |
| Tm | 60.034 | 59.759 | End: 750 |
| Size | 20 | 20 | 132 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAAGGCCATTCCCGGTT | GGGAGGAGGTTTGAGGCTTC | Start: 1513 |
| Tm | 59.886 | 60.035 | End: 1674 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | AAGAAAAGGCCATTCCCGGT | GGGAGGAGGTTTGAGGCTTC | Start: 1512 |
| Tm | 59.886 | 60.035 | End: 1674 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GTTCTCGGATAGGCACCCAG | ACCGGGAATGGCCTTTTCTT | Start: 1327 |
| Tm | 59.895 | 59.886 | End: 1531 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | GTTCTCGGATAGGCACCCAG | AACCGGGAATGGCCTTTTCT | Start: 1327 |
| Tm | 59.895 | 59.886 | End: 1532 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
|---|
| Sequence | ACTCCACAGGCCACAAACAA | CGAAGCTTCTCTCCACGTGA | Start: 619 |
| Tm | 60.034 | 59.759 | End: 750 |
| Size | 20 | 20 | 132 |
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