ssr_primer
Primer Details for TRINITY_DN14219_c1_g2_i10 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACACTCACTCTCACGTGC | ATTGGTGCAATCTGAGCGGA | Start: 32 |
| Tm | 59.969 | 60.035 | End: 164 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | CACACTCACTCTCACGTGCT | ATTGGTGCAATCTGAGCGGA | Start: 33 |
| Tm | 59.969 | 60.035 | End: 164 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CACTCTCACGTGCTCACCTT | ATTGGTGCAATCTGAGCGGA | Start: 39 |
| Tm | 59.967 | 60.035 | End: 164 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TCACGTGCTCACCTTCTTCC | ATTGGTGCAATCTGAGCGGA | Start: 44 |
| Tm | 59.966 | 60.035 | End: 164 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | ACCAACGGCATTGACGAGAT | TCGCCTTTTCACCCTCAGTC | Start: 4860 |
| Tm | 60.036 | 59.965 | End: 4990 |
| Size | 20 | 20 | 131 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACACTCACTCTCACGTGC | ATTGGTGCAATCTGAGCGGA | Start: 32 |
| Tm | 59.969 | 60.035 | End: 164 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | CACACTCACTCTCACGTGCT | ATTGGTGCAATCTGAGCGGA | Start: 33 |
| Tm | 59.969 | 60.035 | End: 164 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | CACTCTCACGTGCTCACCTT | ATTGGTGCAATCTGAGCGGA | Start: 39 |
| Tm | 59.967 | 60.035 | End: 164 |
| Size | 20 | 20 | 126 |
| Primer 4 details |
|---|
| Sequence | TCACGTGCTCACCTTCTTCC | ATTGGTGCAATCTGAGCGGA | Start: 44 |
| Tm | 59.966 | 60.035 | End: 164 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | ACCAACGGCATTGACGAGAT | TCGCCTTTTCACCCTCAGTC | Start: 4860 |
| Tm | 60.036 | 59.965 | End: 4990 |
| Size | 20 | 20 | 131 |
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