ssr_primer
Primer Details for TRINITY_DN14204_c1_g1_i4 |
|---|
| (A)10caaacaaatcatcaatgaattccattttaggcacaaacaagctagaaagagcaaaaaaaatcctaacttcgctatgcaatatgacctttctgttg(T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTGCTTTCGCTAACATC | ATGTTGCATGGCAGGATGGA | Start: 4027 |
| Tm | 59.973 | 60.033 | End: 4177 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCCATCCTGCCATGCAACAT | AGGAGACTGCCCATGAGACT | Start: 4158 |
| Tm | 60.033 | 59.958 | End: 4315 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | ATCGGTGGGTGCTTGTGATT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4470 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | AATCGGTGGGTGCTTGTGAT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4471 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCTGAAGCGAGACCAACACT | GGGTGGCGGATAAATCCCAA | Start: 1429 |
| Tm | 59.966 | 60.106 | End: 1564 |
| Size | 20 | 20 | 136 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTGCTTTCGCTAACATC | ATGTTGCATGGCAGGATGGA | Start: 4027 |
| Tm | 59.973 | 60.033 | End: 4177 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCCATCCTGCCATGCAACAT | AGGAGACTGCCCATGAGACT | Start: 4158 |
| Tm | 60.033 | 59.958 | End: 4315 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | ATCGGTGGGTGCTTGTGATT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4470 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | AATCGGTGGGTGCTTGTGAT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4471 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCTGAAGCGAGACCAACACT | GGGTGGCGGATAAATCCCAA | Start: 1429 |
| Tm | 59.966 | 60.106 | End: 1564 |
| Size | 20 | 20 | 136 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTGCTTTCGCTAACATC | ATGTTGCATGGCAGGATGGA | Start: 4027 |
| Tm | 59.973 | 60.033 | End: 4177 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | TCCATCCTGCCATGCAACAT | AGGAGACTGCCCATGAGACT | Start: 4158 |
| Tm | 60.033 | 59.958 | End: 4315 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | ATCGGTGGGTGCTTGTGATT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4470 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | AGTCTCATGGGCAGTCTCCT | AATCGGTGGGTGCTTGTGAT | Start: 4296 |
| Tm | 59.958 | 59.962 | End: 4471 |
| Size | 20 | 20 | 176 |
| Primer 5 details |
|---|
| Sequence | CCTGAAGCGAGACCAACACT | GGGTGGCGGATAAATCCCAA | Start: 1429 |
| Tm | 59.966 | 60.106 | End: 1564 |
| Size | 20 | 20 | 136 |
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