ssr_primer
Primer Details for TRINITY_DN14175_c2_g2_i6 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTGAGGGTAGCGAAAAG | TCTCCTGCTACCAGCCTCAT | Start: 1081 |
| Tm | 60.036 | 60.032 | End: 1228 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | TGAAACACAACGGGGGTTGA | CCCCTAGCCTGTAAGCATCG | Start: 2691 |
| Tm | 60.034 | 59.966 | End: 2915 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | CAGAAGCAGAGCCATTGGGA | CCCCTAGCCTGTAAGCATCG | Start: 2637 |
| Tm | 60.035 | 59.966 | End: 2915 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATGAGGCTGGTAGCAGGAGA | AGAGAACGCAGAAGGACACG | Start: 1209 |
| Tm | 60.032 | 60.039 | End: 1437 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | TGGCTGAGGGTAGCGAAAAG | GGAACGTGCAGATCCCTGAA | Start: 1081 |
| Tm | 60.036 | 60.036 | End: 1208 |
| Size | 20 | 20 | 128 |
(TTA)5tccgatccaaaaaaaggactccatctacaccatactg(TCCCA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTGAGGGTAGCGAAAAG | TCTCCTGCTACCAGCCTCAT | Start: 1081 |
| Tm | 60.036 | 60.032 | End: 1228 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | TGAAACACAACGGGGGTTGA | CCCCTAGCCTGTAAGCATCG | Start: 2691 |
| Tm | 60.034 | 59.966 | End: 2915 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | CAGAAGCAGAGCCATTGGGA | CCCCTAGCCTGTAAGCATCG | Start: 2637 |
| Tm | 60.035 | 59.966 | End: 2915 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | ATGAGGCTGGTAGCAGGAGA | AGAGAACGCAGAAGGACACG | Start: 1209 |
| Tm | 60.032 | 60.039 | End: 1437 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | TGGCTGAGGGTAGCGAAAAG | GGAACGTGCAGATCCCTGAA | Start: 1081 |
| Tm | 60.036 | 60.036 | End: 1208 |
| Size | 20 | 20 | 128 |
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