ssr_primer
Primer Details for TRINITY_DN14166_c2_g1_i5 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACCATTGTCATCCCCTGA | TCATCAATGACGGACGTGCA | Start: 2182 |
| Tm | 60.034 | 60.038 | End: 2353 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | GCAGCAAGAACGTTGTCTGG | TCATCAATGACGGACGTGCA | Start: 2145 |
| Tm | 60.041 | 60.038 | End: 2353 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TGTTGCCAAAAGCTTCGTGG | TCAGGGGATGACAATGGTGC | Start: 2070 |
| Tm | 59.898 | 60.034 | End: 2201 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | CAAGACGCTCCACAGGGTAG | GCTTGATGAAGACCGGGTCA | Start: 1348 |
| Tm | 60.109 | 60.036 | End: 1579 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CAAGACGCTCCACAGGGTAG | ACCGGGTCATTGTTGCATCT | Start: 1348 |
| Tm | 60.109 | 59.962 | End: 1568 |
| Size | 20 | 20 | 221 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACCATTGTCATCCCCTGA | TCATCAATGACGGACGTGCA | Start: 2182 |
| Tm | 60.034 | 60.038 | End: 2353 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | GCAGCAAGAACGTTGTCTGG | TCATCAATGACGGACGTGCA | Start: 2145 |
| Tm | 60.041 | 60.038 | End: 2353 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TGTTGCCAAAAGCTTCGTGG | TCAGGGGATGACAATGGTGC | Start: 2070 |
| Tm | 59.898 | 60.034 | End: 2201 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | CAAGACGCTCCACAGGGTAG | GCTTGATGAAGACCGGGTCA | Start: 1348 |
| Tm | 60.109 | 60.036 | End: 1579 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CAAGACGCTCCACAGGGTAG | ACCGGGTCATTGTTGCATCT | Start: 1348 |
| Tm | 60.109 | 59.962 | End: 1568 |
| Size | 20 | 20 | 221 |
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