ssr_primer
Primer Details for TRINITY_DN14158_c0_g1_i7 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAACCACCTCGTTTGACAC | AGACAGGATCTTTGCCCAGC | Start: 1453 |
| Tm | 59.971 | 60.035 | End: 1559 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
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| Sequence | AGGTTCAATGGCCGGAAGAG | GTGTCAAACGAGGTGGTTGC | Start: 1326 |
| Tm | 60.035 | 59.971 | End: 1472 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
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| Sequence | ACGCTCGACCTCCAACAAAT | GCCCTCATGTTTTTGCCGTT | Start: 9887 |
| Tm | 59.965 | 59.967 | End: 10042 |
| Size | 20 | 20 | 156 |
| Primer 4 details |
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| Sequence | AGGTTCAATGGCCGGAAGAG | AGACAGGATCTTTGCCCAGC | Start: 1326 |
| Tm | 60.035 | 60.035 | End: 1559 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | GCTAAGCCAGTGGAACCAGT | AATGACGCCCTCAAGTCTGG | Start: 2155 |
| Tm | 59.963 | 60.036 | End: 2306 |
| Size | 20 | 20 | 152 |
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