Primer Details for TRINITY_DN14154_c1_g3_i1 | |||
|---|---|---|---|
| (T)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGCTATCTACTTTCCAAACGAGA | AGTTCATTCCGGCAAAAACTCG | Start: 162 |
| Tm | 59.582 | 60.031 | End: 261 |
| Size | 25 | 22 | 100 |
| Primer 2 details | |||
| Sequence | TCTGCTATCTACTTTCCAAACGAG | AGTTCATTCCGGCAAAAACTCG | Start: 162 |
| Tm | 58.348 | 60.031 | End: 261 |
| Size | 24 | 22 | 100 |
| Primer 3 details | |||
| Sequence | TCTGCTATCTACTTTCCAAACGA | AGTTCATTCCGGCAAAAACTCG | Start: 162 |
| Tm | 57.345 | 60.031 | End: 261 |
| Size | 23 | 22 | 100 |
| Primer 4 details | |||
| Sequence | TTCTGCTATCTACTTTCCAAACGA | AGTTCATTCCGGCAAAAACTCG | Start: 161 |
| Tm | 57.967 | 60.031 | End: 261 |
| Size | 24 | 22 | 101 |
| Primer 5 details | |||
| Sequence | TTCTGCTATCTACTTTCCAAACGA | GTTCATTCCGGCAAAAACTCGA | Start: 161 |
| Tm | 57.967 | 60.032 | End: 260 |
| Size | 24 | 22 | 100 |
