Primer Details for TRINITY_DN14129_c4_g1_i1 |
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| (TTG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGAAACATCAATACACTTGACCC | AGCTCGCCTCATACTTCAACC | Start: 35 |
| Tm | 59.876 | 60.135 | End: 152 |
| Size | 25 | 21 | 118 |
| Primer 2 details |
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| Sequence | ACATCAATACACTTGACCCTACAGT | AGCTCGCCTCATACTTCAACC | Start: 41 |
| Tm | 59.752 | 60.135 | End: 152 |
| Size | 25 | 21 | 112 |
| Primer 3 details |
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| Sequence | GGAGAAACATCAATACACTTGACCC | GCTCGCCTCATACTTCAACC | Start: 35 |
| Tm | 59.876 | 58.709 | End: 151 |
| Size | 25 | 20 | 117 |
| Primer 4 details |
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| Sequence | ACATCAATACACTTGACCCTACAGT | GCTCGCCTCATACTTCAACC | Start: 41 |
| Tm | 59.752 | 58.709 | End: 151 |
| Size | 25 | 20 | 111 |
| Primer 5 details |
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| Sequence | GGAGAAACATCAATACACTTGACCC | CTCGCCTCATACTTCAACCCT | Start: 35 |
| Tm | 59.876 | 59.517 | End: 150 |
| Size | 25 | 21 | 116 |
