ssr_primer
Primer Details for TRINITY_DN14122_c2_g5_i2 |
|---|
| (TA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAGCTCCTCAACGATGGCG | CCTGTCCGACTTACACCACC | Start: 3008 |
| Tm | 59.967 | 60.038 | End: 3186 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | TCGTCGTCTCGTCCACTTTG | CGCCATCGTTGAGGAGCTAT | Start: 2832 |
| Tm | 60.041 | 59.967 | End: 3027 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | GGTGGTGTAAGTCGGACAGG | CAGCTCTCCACCACCAACAT | Start: 3167 |
| Tm | 60.038 | 59.963 | End: 3293 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GCGGAACCCAGAAACGAAAC | CAGCTCTCCACCACCAACAT | Start: 3025 |
| Tm | 60.041 | 59.963 | End: 3293 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | GCGGAACCCAGAAACGAAAC | CCTGTCCGACTTACACCACC | Start: 3025 |
| Tm | 60.041 | 60.038 | End: 3186 |
| Size | 20 | 20 | 162 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATAGCTCCTCAACGATGGCG | CCTGTCCGACTTACACCACC | Start: 3008 |
| Tm | 59.967 | 60.038 | End: 3186 |
| Size | 20 | 20 | 179 |
| Primer 2 details |
|---|
| Sequence | TCGTCGTCTCGTCCACTTTG | CGCCATCGTTGAGGAGCTAT | Start: 2832 |
| Tm | 60.041 | 59.967 | End: 3027 |
| Size | 20 | 20 | 196 |
| Primer 3 details |
|---|
| Sequence | GGTGGTGTAAGTCGGACAGG | CAGCTCTCCACCACCAACAT | Start: 3167 |
| Tm | 60.038 | 59.963 | End: 3293 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | GCGGAACCCAGAAACGAAAC | CAGCTCTCCACCACCAACAT | Start: 3025 |
| Tm | 60.041 | 59.963 | End: 3293 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | GCGGAACCCAGAAACGAAAC | CCTGTCCGACTTACACCACC | Start: 3025 |
| Tm | 60.041 | 60.038 | End: 3186 |
| Size | 20 | 20 | 162 |
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