Primer Details for TRINITY_DN14115_c2_g1_i1 | |||
|---|---|---|---|
| (A)20: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCATGGTAATGTAAGGACA | GGAGACCGTTTGAATCCGCT | Start: 45 |
| Tm | 57.827 | 60.391 | End: 204 |
| Size | 23 | 20 | 160 |
| Primer 2 details | |||
| Sequence | TGCTTCATGGTAATGTAAGGACAG | GGAGACCGTTTGAATCCGCT | Start: 45 |
| Tm | 58.815 | 60.391 | End: 204 |
| Size | 24 | 20 | 160 |
| Primer 3 details | |||
| Sequence | GCTTCATGGTAATGTAAGGACAGT | GGAGACCGTTTGAATCCGCT | Start: 46 |
| Tm | 58.814 | 60.391 | End: 204 |
| Size | 24 | 20 | 159 |
| Primer 4 details | |||
| Sequence | TGCTTCATGGTAATGTAAGGACAGT | GGAGACCGTTTGAATCCGCT | Start: 45 |
| Tm | 60.28 | 60.391 | End: 204 |
| Size | 25 | 20 | 160 |
| Primer 5 details | |||
| Sequence | AAACTTCAACTTTGCTTCATGGT | GGAGACCGTTTGAATCCGCT | Start: 33 |
| Tm | 57.657 | 60.391 | End: 204 |
| Size | 23 | 20 | 172 |
