ssr_primer
Primer Details for TRINITY_DN14088_c3_g1_i1 |
|---|
| (ATTT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCTCTGCTTGCATGTGA | CCCAAGTTACGTGGCTGGAA | Start: 63 |
| Tm | 60.251 | 60.251 | End: 189 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | ACTGCTCTGCTTGCATGTGA | TACCCAAGTTACGTGGCTGG | Start: 63 |
| Tm | 60.251 | 59.678 | End: 191 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | GGGGGAGGGAGGAGTAAAGT | ACCCCATGCGTTCAAATGGT | Start: 448 |
| Tm | 59.956 | 60.542 | End: 592 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | GGGGGAGGGAGGAGTAAAGT | AACCCCATGCGTTCAAATGG | Start: 448 |
| Tm | 59.956 | 59.392 | End: 593 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | ATTTTGAGGGGGAGGGAGGA | ACCCCATGCGTTCAAATGGT | Start: 441 |
| Tm | 59.877 | 60.542 | End: 592 |
| Size | 20 | 20 | 152 |
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