ssr_primer
Primer Details for TRINITY_DN14076_c5_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAACAGGTTTTGCCTCC | AAGAGGGAGGCCATTTGACG | Start: 243 |
| Tm | 60.469 | 60.035 | End: 374 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
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| Sequence | CCCAACAGGTTTTGCCTCCT | AAGAGGGAGGCCATTTGACG | Start: 244 |
| Tm | 60.47 | 60.035 | End: 374 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | ACAGGTTTTGCCTCCTCCAA | AAGAGGGAGGCCATTTGACG | Start: 248 |
| Tm | 59.444 | 60.035 | End: 374 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
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| Sequence | AACAGGTTTTGCCTCCTCCA | AAGAGGGAGGCCATTTGACG | Start: 247 |
| Tm | 59.444 | 60.035 | End: 374 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
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| Sequence | TCCCAACAGGTTTTGCCTCC | CATTTGACGGGGCATCTTGC | Start: 243 |
| Tm | 60.469 | 60.179 | End: 363 |
| Size | 20 | 20 | 121 |
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