ssr_primer
Primer Details for TRINITY_DN14076_c4_g5_i2 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATCCGTCTCCCCAACA | CGGGTGGATTCTGTGGGAAA | Start: 233 |
| Tm | 60.037 | 59.962 | End: 375 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | TTTCGGGTGGATTCTGTGGG | Start: 233 |
| Tm | 60.037 | 59.962 | End: 378 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | GAGGGAGAGGGAGAATCGGT | Start: 233 |
| Tm | 60.037 | 60.106 | End: 502 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CCCACAGAATCCACCCGAAA | GAGGGAGAGGGAGAATCGGT | Start: 359 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCACAGAATCCACCCG | GAGGGAGAGGGAGAATCGGT | Start: 356 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 147 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATCCGTCTCCCCAACA | CGGGTGGATTCTGTGGGAAA | Start: 233 |
| Tm | 60.037 | 59.962 | End: 375 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | TTTCGGGTGGATTCTGTGGG | Start: 233 |
| Tm | 60.037 | 59.962 | End: 378 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | GAGGGAGAGGGAGAATCGGT | Start: 233 |
| Tm | 60.037 | 60.106 | End: 502 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CCCACAGAATCCACCCGAAA | GAGGGAGAGGGAGAATCGGT | Start: 359 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCACAGAATCCACCCG | GAGGGAGAGGGAGAATCGGT | Start: 356 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 147 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATCCGTCTCCCCAACA | CGGGTGGATTCTGTGGGAAA | Start: 233 |
| Tm | 60.037 | 59.962 | End: 375 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | TTTCGGGTGGATTCTGTGGG | Start: 233 |
| Tm | 60.037 | 59.962 | End: 378 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | GAGGGAGAGGGAGAATCGGT | Start: 233 |
| Tm | 60.037 | 60.106 | End: 502 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CCCACAGAATCCACCCGAAA | GAGGGAGAGGGAGAATCGGT | Start: 359 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCACAGAATCCACCCG | GAGGGAGAGGGAGAATCGGT | Start: 356 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 147 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATCCGTCTCCCCAACA | CGGGTGGATTCTGTGGGAAA | Start: 233 |
| Tm | 60.037 | 59.962 | End: 375 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | TTTCGGGTGGATTCTGTGGG | Start: 233 |
| Tm | 60.037 | 59.962 | End: 378 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | CGAAATCCGTCTCCCCAACA | GAGGGAGAGGGAGAATCGGT | Start: 233 |
| Tm | 60.037 | 60.106 | End: 502 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | CCCACAGAATCCACCCGAAA | GAGGGAGAGGGAGAATCGGT | Start: 359 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCACAGAATCCACCCG | GAGGGAGAGGGAGAATCGGT | Start: 356 |
| Tm | 59.962 | 60.106 | End: 502 |
| Size | 20 | 20 | 147 |
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