ssr_primer
Primer Details for TRINITY_DN14059_c1_g6_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTGCTAGGACGACACAC | TTCCAGCGGAAACAGGTACC | Start: 999 |
| Tm | 60.038 | 59.964 | End: 1257 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | GGTACCTGTTTCCGCTGGAA | TGGGACGCAAGAAGATCGAG | Start: 1238 |
| Tm | 59.964 | 59.827 | End: 1426 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GGTACCTGTTTCCGCTGGAA | GGACGCAAGAAGATCGAGGT | Start: 1238 |
| Tm | 59.964 | 59.826 | End: 1424 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | CCAACCTGAACGTCGCAAAG | TGGGACGCAAGAAGATCGAG | Start: 1297 |
| Tm | 60.041 | 59.827 | End: 1426 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | CCAACCTGAACGTCGCAAAG | GGACGCAAGAAGATCGAGGT | Start: 1297 |
| Tm | 60.041 | 59.826 | End: 1424 |
| Size | 20 | 20 | 128 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTGCTAGGACGACACAC | TTCCAGCGGAAACAGGTACC | Start: 999 |
| Tm | 60.038 | 59.964 | End: 1257 |
| Size | 20 | 20 | 259 |
| Primer 2 details |
|---|
| Sequence | GGTACCTGTTTCCGCTGGAA | TGGGACGCAAGAAGATCGAG | Start: 1238 |
| Tm | 59.964 | 59.827 | End: 1426 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GGTACCTGTTTCCGCTGGAA | GGACGCAAGAAGATCGAGGT | Start: 1238 |
| Tm | 59.964 | 59.826 | End: 1424 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | CCAACCTGAACGTCGCAAAG | TGGGACGCAAGAAGATCGAG | Start: 1297 |
| Tm | 60.041 | 59.827 | End: 1426 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | CCAACCTGAACGTCGCAAAG | GGACGCAAGAAGATCGAGGT | Start: 1297 |
| Tm | 60.041 | 59.826 | End: 1424 |
| Size | 20 | 20 | 128 |
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