Primer Details for TRINITY_DN14048_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAA | Start: 324 |
| Tm | 58.83 | 58.116 | End: 534 |
| Size | 25 | 20 | 211 |
| Primer 2 details |
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| Sequence | AGCCAAACAAAACATTTACAAGTGT | TTCACACACACACACACACA | Start: 324 |
| Tm | 58.83 | 58.116 | End: 535 |
| Size | 25 | 20 | 212 |
| Primer 3 details |
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| Sequence | GCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAA | Start: 325 |
| Tm | 57.584 | 58.116 | End: 534 |
| Size | 24 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | GCCAAACAAAACATTTACAAGTGT | TTCACACACACACACACACA | Start: 325 |
| Tm | 57.584 | 58.116 | End: 535 |
| Size | 24 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | AGCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAAA | Start: 324 |
| Tm | 58.83 | 58.778 | End: 534 |
| Size | 25 | 21 | 211 |
(TG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAA | Start: 324 |
| Tm | 58.83 | 58.116 | End: 534 |
| Size | 25 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | AGCCAAACAAAACATTTACAAGTGT | TTCACACACACACACACACA | Start: 324 |
| Tm | 58.83 | 58.116 | End: 535 |
| Size | 25 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | GCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAA | Start: 325 |
| Tm | 57.584 | 58.116 | End: 534 |
| Size | 24 | 20 | 210 |
| Primer 4 details |
|---|
| Sequence | GCCAAACAAAACATTTACAAGTGT | TTCACACACACACACACACA | Start: 325 |
| Tm | 57.584 | 58.116 | End: 535 |
| Size | 24 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | AGCCAAACAAAACATTTACAAGTGT | TCACACACACACACACACAAA | Start: 324 |
| Tm | 58.83 | 58.778 | End: 534 |
| Size | 25 | 21 | 211 |
