Primer Details for TRINITY_DN14048_c1_g1_i1 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATACTATCTATTGTGGTCCATCGA | CCTATCAAAGAAATCCCTAAAGTCTGT | Start: 86 |
| Tm | 58.824 | 58.95 | End: 252 |
| Size | 26 | 27 | 167 |
| Primer 2 details |
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| Sequence | TGGAGACGGTATCAAAGATACTATCT | CCTATCAAAGAAATCCCTAAAGTCTGT | Start: 71 |
| Tm | 58.768 | 58.95 | End: 252 |
| Size | 26 | 27 | 182 |
| Primer 3 details |
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| Sequence | GATACTATCTATTGTGGTCCATCGA | CCTATCAAAGAAATCCCTAAAGTCTGT | Start: 87 |
| Tm | 57.608 | 58.95 | End: 252 |
| Size | 25 | 27 | 166 |
| Primer 4 details |
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| Sequence | AGATACTATCTATTGTGGTCCATCGA | TCCTATCAAAGAAATCCCTAAAGTCTG | Start: 86 |
| Tm | 58.824 | 58.732 | End: 253 |
| Size | 26 | 27 | 168 |
| Primer 5 details |
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| Sequence | ACTATCTATTGTGGTCCATCGATAG | CCTATCAAAGAAATCCCTAAAGTCTGT | Start: 90 |
| Tm | 57.602 | 58.95 | End: 252 |
| Size | 25 | 27 | 163 |
