ssr_primer
Primer Details for TRINITY_DN14048_c0_g6_i3 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAAAAATTGACGCCTCGC | GAGGTGAAGGAGTCAAGGGC | Start: 12 |
| Tm | 60.04 | 60.036 | End: 166 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GCCCTTGACTCCTTCACCTC | ATCGGATGTACCTTGTCGGC | Start: 147 |
| Tm | 60.036 | 59.896 | End: 409 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | CTTCGCCGTCACCAACAAAG | TCCTGACGCGATTCTGTAGC | Start: 577 |
| Tm | 60.041 | 59.899 | End: 680 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | CCCGTAATCGTCATCGGTGT | CTTTGTTGGTGACGGCGAAG | Start: 431 |
| Tm | 59.898 | 60.041 | End: 596 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | GCCCTTGACTCCTTCACCTC | ATGTACCTTGTCGGCTGAGC | Start: 147 |
| Tm | 60.036 | 60.108 | End: 404 |
| Size | 20 | 20 | 258 |
(ATA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAAAAATTGACGCCTCGC | GAGGTGAAGGAGTCAAGGGC | Start: 12 |
| Tm | 60.04 | 60.036 | End: 166 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GCCCTTGACTCCTTCACCTC | ATCGGATGTACCTTGTCGGC | Start: 147 |
| Tm | 60.036 | 59.896 | End: 409 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | CTTCGCCGTCACCAACAAAG | TCCTGACGCGATTCTGTAGC | Start: 577 |
| Tm | 60.041 | 59.899 | End: 680 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | CCCGTAATCGTCATCGGTGT | CTTTGTTGGTGACGGCGAAG | Start: 431 |
| Tm | 59.898 | 60.041 | End: 596 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | GCCCTTGACTCCTTCACCTC | ATGTACCTTGTCGGCTGAGC | Start: 147 |
| Tm | 60.036 | 60.108 | End: 404 |
| Size | 20 | 20 | 258 |
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