ssr_primer
Primer Details for TRINITY_DN14038_c0_g2_i19 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTTCCGAGCTTCTGCCTC | CCGCTGTGTCTGTGAGTCTT | Start: 3476 |
| Tm | 60.037 | 59.967 | End: 3709 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | ACCTACAAAGACAGGTGGCG | AGAATGGCGTGACGTGCTAA | Start: 5718 |
| Tm | 59.965 | 60.038 | End: 5960 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | TCTGAATTCCCCTTTCCCGC | TTGAATAAGCCCCAGCCGTT | Start: 3084 |
| Tm | 60.034 | 59.961 | End: 3303 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | TACAAAGACAGGTGGCGCAT | AGAATGGCGTGACGTGCTAA | Start: 5721 |
| Tm | 59.964 | 60.038 | End: 5960 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | AACGGCTGGGGCTTATTCAA | GAGGCAGAAGCTCGGAAACT | Start: 3284 |
| Tm | 59.961 | 60.037 | End: 3495 |
| Size | 20 | 20 | 212 |
(AT)7acac(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTTCCGAGCTTCTGCCTC | CCGCTGTGTCTGTGAGTCTT | Start: 3476 |
| Tm | 60.037 | 59.967 | End: 3709 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
|---|
| Sequence | ACCTACAAAGACAGGTGGCG | AGAATGGCGTGACGTGCTAA | Start: 5718 |
| Tm | 59.965 | 60.038 | End: 5960 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | TCTGAATTCCCCTTTCCCGC | TTGAATAAGCCCCAGCCGTT | Start: 3084 |
| Tm | 60.034 | 59.961 | End: 3303 |
| Size | 20 | 20 | 220 |
| Primer 4 details |
|---|
| Sequence | TACAAAGACAGGTGGCGCAT | AGAATGGCGTGACGTGCTAA | Start: 5721 |
| Tm | 59.964 | 60.038 | End: 5960 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | AACGGCTGGGGCTTATTCAA | GAGGCAGAAGCTCGGAAACT | Start: 3284 |
| Tm | 59.961 | 60.037 | End: 3495 |
| Size | 20 | 20 | 212 |
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