ssr_primer
Primer Details for TRINITY_DN14037_c0_g1_i5 |
|---|
| (CT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAAGAGGAGGAGGAGCGA | CGCTGCGACATGACAATCAG | Start: 4290 |
| Tm | 59.96 | 59.974 | End: 4467 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GCACGTCCATTCCAGGTGTA | GCATGTTCATGCATCCGGTC | Start: 3267 |
| Tm | 60.037 | 59.97 | End: 3391 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCACGTCCATTCCAGGTGTA | AGCTTGGATGGCAGCAGATT | Start: 3267 |
| Tm | 60.037 | 60.033 | End: 3425 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GGAACTGCAATCCTCTGGCT | TACACCTGGAATGGACGTGC | Start: 3009 |
| Tm | 60.035 | 60.037 | End: 3286 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | AGGAACTGCAATCCTCTGGC | TACACCTGGAATGGACGTGC | Start: 3008 |
| Tm | 60.035 | 60.037 | End: 3286 |
| Size | 20 | 20 | 279 |
(A)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAAGAGGAGGAGGAGCGA | CGCTGCGACATGACAATCAG | Start: 4290 |
| Tm | 59.96 | 59.974 | End: 4467 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | GCACGTCCATTCCAGGTGTA | GCATGTTCATGCATCCGGTC | Start: 3267 |
| Tm | 60.037 | 59.97 | End: 3391 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCACGTCCATTCCAGGTGTA | AGCTTGGATGGCAGCAGATT | Start: 3267 |
| Tm | 60.037 | 60.033 | End: 3425 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | GGAACTGCAATCCTCTGGCT | TACACCTGGAATGGACGTGC | Start: 3009 |
| Tm | 60.035 | 60.037 | End: 3286 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | AGGAACTGCAATCCTCTGGC | TACACCTGGAATGGACGTGC | Start: 3008 |
| Tm | 60.035 | 60.037 | End: 3286 |
| Size | 20 | 20 | 279 |
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