ssr_primer
Primer Details for TRINITY_DN14025_c0_g7_i3 |
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| (AAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTGCTAAGATGCAAGCTG | GCTTTCCGGACGACTTACCA | Start: 673 |
| Tm | 59.972 | 60.038 | End: 937 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
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| Sequence | CGCTGCTAAGATGCAAGCTG | TTGCTGATGTGCTGGTGGAT | Start: 673 |
| Tm | 59.972 | 59.961 | End: 897 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
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| Sequence | AAGATGCAAGCTGGACCCTC | GCTTTCCGGACGACTTACCA | Start: 680 |
| Tm | 60.035 | 60.038 | End: 937 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | AAGATGCAAGCTGGACCCTC | TTGCTGATGTGCTGGTGGAT | Start: 680 |
| Tm | 60.035 | 59.961 | End: 897 |
| Size | 20 | 20 | 218 |
| Primer 5 details |
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| Sequence | TGGTAAGTCGTCCGGAAAGC | GAGGAGGCCGAAACGAAAGA | Start: 918 |
| Tm | 60.038 | 60.038 | End: 1176 |
| Size | 20 | 20 | 259 |
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