ssr_primer
Primer Details for TRINITY_DN14010_c1_g3_i3 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTTCTCTGCCCTGGTTT | GTTGATGCAAGTTGCTGGCA | Start: 239 |
| Tm | 59.965 | 59.968 | End: 460 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GCCAACAAACGGTCGAGTTC | GTTGATGCAAGTTGCTGGCA | Start: 226 |
| Tm | 60.042 | 59.968 | End: 460 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | CCTCCGACCAATCCGATTCC | GTTGATGCAAGTTGCTGGCA | Start: 316 |
| Tm | 60.25 | 59.968 | End: 460 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | TGTCGAACGGCAGATGATGT | ACGTTTCCTGCTCTAGCCAC | Start: 1422 |
| Tm | 59.754 | 60.037 | End: 1557 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGTCGAACGGCAGATGATGT | CGTTTCCTGCTCTAGCCACA | Start: 1422 |
| Tm | 59.754 | 60.037 | End: 1556 |
| Size | 20 | 20 | 135 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTTCTCTGCCCTGGTTT | GTTGATGCAAGTTGCTGGCA | Start: 239 |
| Tm | 59.965 | 59.968 | End: 460 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | GCCAACAAACGGTCGAGTTC | GTTGATGCAAGTTGCTGGCA | Start: 226 |
| Tm | 60.042 | 59.968 | End: 460 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | CCTCCGACCAATCCGATTCC | GTTGATGCAAGTTGCTGGCA | Start: 316 |
| Tm | 60.25 | 59.968 | End: 460 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
|---|
| Sequence | TGTCGAACGGCAGATGATGT | ACGTTTCCTGCTCTAGCCAC | Start: 1422 |
| Tm | 59.754 | 60.037 | End: 1557 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGTCGAACGGCAGATGATGT | CGTTTCCTGCTCTAGCCACA | Start: 1422 |
| Tm | 59.754 | 60.037 | End: 1556 |
| Size | 20 | 20 | 135 |
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