ssr_primer
Primer Details for TRINITY_DN14009_c2_g3_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGGCCCTCCCTCATCAT | TGGGACAAACAGTACGGTCG | Start: 535 |
| Tm | 60.029 | 59.968 | End: 673 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | AGAAGGCCCTCCCTCATCAT | ACCTGACTCGGTCAATGCAG | Start: 535 |
| Tm | 60.029 | 60.037 | End: 646 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
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| Sequence | CCAGCAGCTCCAGTCTCAAA | TCTCAGATGCCGGATTACGC | Start: 1363 |
| Tm | 59.964 | 59.968 | End: 1500 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
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| Sequence | CCAGCAGCTCCAGTCTCAAA | AAGGAATCGAACGTGCCCAT | Start: 1363 |
| Tm | 59.964 | 60.035 | End: 1464 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | CCAGCAGCTCCAGTCTCAAA | CCGGATTACGCCTTGCAAAG | Start: 1363 |
| Tm | 59.964 | 59.901 | End: 1491 |
| Size | 20 | 20 | 129 |
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