ssr_primer
Primer Details for TRINITY_DN14002_c0_g1_i6 |
|---|
| (A)21: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTTTTGGGATTTGGGCC | TGCGTTTGGATCCTCAAGCT | Start: 250 |
| Tm | 59.964 | 59.963 | End: 387 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | TTTGGGCCGTGAGAGATCAC | TGCGTTTGGATCCTCAAGCT | Start: 262 |
| Tm | 60.036 | 59.963 | End: 387 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | GGCCGTGAGAGATCACACAA | TGCGTTTGGATCCTCAAGCT | Start: 266 |
| Tm | 60.037 | 59.963 | End: 387 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACAGCCGGTGAAAACTAGGG | ATCCCAAACCCTCGAAACCC | Start: 324 |
| Tm | 59.964 | 59.961 | End: 583 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | AGCTTGAGGATCCAAACGCA | ATCCCAAACCCTCGAAACCC | Start: 368 |
| Tm | 59.963 | 59.961 | End: 583 |
| Size | 20 | 20 | 216 |
(GAA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTTTTGGGATTTGGGCC | TGCGTTTGGATCCTCAAGCT | Start: 250 |
| Tm | 59.964 | 59.963 | End: 387 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | TTTGGGCCGTGAGAGATCAC | TGCGTTTGGATCCTCAAGCT | Start: 262 |
| Tm | 60.036 | 59.963 | End: 387 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | GGCCGTGAGAGATCACACAA | TGCGTTTGGATCCTCAAGCT | Start: 266 |
| Tm | 60.037 | 59.963 | End: 387 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACAGCCGGTGAAAACTAGGG | ATCCCAAACCCTCGAAACCC | Start: 324 |
| Tm | 59.964 | 59.961 | End: 583 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | AGCTTGAGGATCCAAACGCA | ATCCCAAACCCTCGAAACCC | Start: 368 |
| Tm | 59.963 | 59.961 | End: 583 |
| Size | 20 | 20 | 216 |
(TC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTTTTGGGATTTGGGCC | TGCGTTTGGATCCTCAAGCT | Start: 250 |
| Tm | 59.964 | 59.963 | End: 387 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | TTTGGGCCGTGAGAGATCAC | TGCGTTTGGATCCTCAAGCT | Start: 262 |
| Tm | 60.036 | 59.963 | End: 387 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | GGCCGTGAGAGATCACACAA | TGCGTTTGGATCCTCAAGCT | Start: 266 |
| Tm | 60.037 | 59.963 | End: 387 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | ACAGCCGGTGAAAACTAGGG | ATCCCAAACCCTCGAAACCC | Start: 324 |
| Tm | 59.964 | 59.961 | End: 583 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | AGCTTGAGGATCCAAACGCA | ATCCCAAACCCTCGAAACCC | Start: 368 |
| Tm | 59.963 | 59.961 | End: 583 |
| Size | 20 | 20 | 216 |
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