Primer Details for TRINITY_DN14000_c2_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGATAGAAACGGATAAACTTCT | TTTGATTGGTCTATCGCTAGCT | Start: 67 |
| Tm | 58.057 | 57.327 | End: 285 |
| Size | 25 | 22 | 219 |
| Primer 2 details |
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| Sequence | TTGGTGATAGAAACGGATAAACTTCT | TTTGATTGGTCTATCGCTAGCT | Start: 66 |
| Tm | 58.608 | 57.327 | End: 285 |
| Size | 26 | 22 | 220 |
| Primer 3 details |
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| Sequence | TGGTGATAGAAACGGATAAACTTCT | ATTTGATTGGTCTATCGCTAGCT | Start: 67 |
| Tm | 58.057 | 57.779 | End: 286 |
| Size | 25 | 23 | 220 |
| Primer 4 details |
|---|
| Sequence | TTGGTGATAGAAACGGATAAACTTC | TTTGATTGGTCTATCGCTAGCT | Start: 66 |
| Tm | 57.395 | 57.327 | End: 285 |
| Size | 25 | 22 | 220 |
| Primer 5 details |
|---|
| Sequence | TGGTGATAGAAACGGATAAACTTCT | AATTTGATTGGTCTATCGCTAGCT | Start: 67 |
| Tm | 58.057 | 58.385 | End: 287 |
| Size | 25 | 24 | 221 |
