ssr_primer
Primer Details for TRINITY_DN13999_c3_g4_i1 |
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| (T)10cttgagcaaaacaaattttattgtgaaagaatgaaagaacaagggcatgcaacacaaaaagtcagcccaccaaaacaccccat(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTACTGCCCACCGAGAACC | TGATGGGCATTGACTCGACC | Start: 1253 |
| Tm | 59.964 | 60.108 | End: 1420 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
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| Sequence | CATTGCAGCGGAAGTCGAAG | TGATGGGCATTGACTCGACC | Start: 1191 |
| Tm | 59.904 | 60.108 | End: 1420 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
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| Sequence | GAAGCCTCAAGCCTCTCCTC | GGATCAGCTTGGCCTTGAGT | Start: 1432 |
| Tm | 59.822 | 60.035 | End: 1703 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
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| Sequence | GAAGCCTCAAGCCTCTCCTC | TGGATCAGCTTGGCCTTGAG | Start: 1432 |
| Tm | 59.822 | 60.035 | End: 1704 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
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| Sequence | GGGCCCCAAAGGAGAGAATC | TCCAGAGGAGGAGTCCATCG | Start: 210 |
| Tm | 60.106 | 60.106 | End: 408 |
| Size | 20 | 20 | 199 |
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