ssr_primer
Primer Details for TRINITY_DN13993_c0_g10_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGATCCACGGTGTTCTCG | TCGGAAGCGCGGATAAATGT | Start: 145 |
| Tm | 59.824 | 60.179 | End: 283 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | CCACGCCTCATCTGTGTCAA | CGAGAACACCGTGGATCCAT | Start: 1 |
| Tm | 60.321 | 59.824 | End: 164 |
| Size | 20 | 20 | 164 |
| Primer 3 details |
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| Sequence | ATGGATCCACGGTGTTCTCG | CTCGGAAGCGCGGATAAATG | Start: 145 |
| Tm | 59.824 | 59.491 | End: 284 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
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| Sequence | ATGGATCCACGGTGTTCTCG | CCTCGGAAGCGCGGATAAAT | Start: 145 |
| Tm | 59.824 | 60.6 | End: 285 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
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| Sequence | GTGGGGGCCTATCAGTTCAA | TCGGAAGCGCGGATAAATGT | Start: 164 |
| Tm | 59.376 | 60.179 | End: 283 |
| Size | 20 | 20 | 120 |
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