ssr_primer
Primer Details for TRINITY_DN13992_c4_g1_i14 |
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| (AG)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCCGTCGAGTGGAGAGGA | CAACCCACCTCAAAAGTGCG | Start: 561 |
| Tm | 60.034 | 59.969 | End: 673 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | AATCCGTCGAGTGGAGAGGA | GCTGGGTGCTTCACAAAAGG | Start: 561 |
| Tm | 60.034 | 59.967 | End: 717 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
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| Sequence | GGCTGTCTTCTGGAAACGGA | ACAGTTGATCAAGCGCAGGA | Start: 1743 |
| Tm | 59.965 | 59.965 | End: 1963 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
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| Sequence | AGGCTGTCTTCTGGAAACGG | ACAGTTGATCAAGCGCAGGA | Start: 1742 |
| Tm | 59.965 | 59.965 | End: 1963 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
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| Sequence | TTGGGGGATTTGGAGAACGG | GTAGGAGGACGCAACCCAAA | Start: 2846 |
| Tm | 59.961 | 59.964 | End: 2995 |
| Size | 20 | 20 | 150 |
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