ssr_primer
Primer Details for TRINITY_DN13991_c3_g9_i1 |
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| (T)15gttttttgtatcccattgtattctttc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGTTGGCTCAGCAGAGG | GCACGACCTTGCCATCAAAG | Start: 222 |
| Tm | 59.964 | 60.11 | End: 323 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | TCTTGTTGGCTCAGCAGAGG | TAATGTGCACGACCTTGCCA | Start: 222 |
| Tm | 59.964 | 60.251 | End: 329 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | TCTTGTTGGCTCAGCAGAGG | TTCCTTTCCTCTCCCTCCCC | Start: 222 |
| Tm | 59.964 | 60.253 | End: 351 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | TGGTTCGGGGAGATCTTCCT | TTCCTTTCCTCTCCCTCCCC | Start: 252 |
| Tm | 59.957 | 60.253 | End: 351 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
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| Sequence | TGTTGACTTGGTTCGGGGAG | TTCCTTTCCTCTCCCTCCCC | Start: 244 |
| Tm | 59.892 | 60.253 | End: 351 |
| Size | 20 | 20 | 108 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGTTGGCTCAGCAGAGG | GCACGACCTTGCCATCAAAG | Start: 222 |
| Tm | 59.964 | 60.11 | End: 323 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TCTTGTTGGCTCAGCAGAGG | TAATGTGCACGACCTTGCCA | Start: 222 |
| Tm | 59.964 | 60.251 | End: 329 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | TCTTGTTGGCTCAGCAGAGG | TTCCTTTCCTCTCCCTCCCC | Start: 222 |
| Tm | 59.964 | 60.253 | End: 351 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | TGGTTCGGGGAGATCTTCCT | TTCCTTTCCTCTCCCTCCCC | Start: 252 |
| Tm | 59.957 | 60.253 | End: 351 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | TGTTGACTTGGTTCGGGGAG | TTCCTTTCCTCTCCCTCCCC | Start: 244 |
| Tm | 59.892 | 60.253 | End: 351 |
| Size | 20 | 20 | 108 |
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