ssr_primer
Primer Details for TRINITY_DN13991_c3_g5_i2 |
|---|
| (CA)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGACCCACGTTTCTGCTC | TGGAGGGGGAGACATTGTCA | Start: 679 |
| Tm | 59.966 | 60.179 | End: 852 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGCTCACATTACCCGCCATT | TGGAGGGGGAGACATTGTCA | Start: 694 |
| Tm | 60.035 | 60.179 | End: 852 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | AAGGGGGTAATAGCTGCTGC | GAGCAGAAACGTGGGTCTGA | Start: 527 |
| Tm | 59.816 | 59.966 | End: 698 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | AAGGGGGTAATAGCTGCTGC | AATGGCGGGTAATGTGAGCA | Start: 527 |
| Tm | 59.816 | 60.035 | End: 713 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | GCAGTGGAAAAAGATCGCCG | CCGTCTACAAGCGTGACTGT | Start: 955 |
| Tm | 60.179 | 60.039 | End: 1146 |
| Size | 20 | 20 | 192 |
(AAT)7aaaaacagt(GA)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGACCCACGTTTCTGCTC | TGGAGGGGGAGACATTGTCA | Start: 679 |
| Tm | 59.966 | 60.179 | End: 852 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGCTCACATTACCCGCCATT | TGGAGGGGGAGACATTGTCA | Start: 694 |
| Tm | 60.035 | 60.179 | End: 852 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | AAGGGGGTAATAGCTGCTGC | GAGCAGAAACGTGGGTCTGA | Start: 527 |
| Tm | 59.816 | 59.966 | End: 698 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | AAGGGGGTAATAGCTGCTGC | AATGGCGGGTAATGTGAGCA | Start: 527 |
| Tm | 59.816 | 60.035 | End: 713 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | GCAGTGGAAAAAGATCGCCG | CCGTCTACAAGCGTGACTGT | Start: 955 |
| Tm | 60.179 | 60.039 | End: 1146 |
| Size | 20 | 20 | 192 |
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