ssr_primer
Primer Details for TRINITY_DN13988_c1_g7_i11 |
|---|
| (ATC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATGGGGCTGATGAGTCT | AGCTGCTTCTCCACCCAATC | Start: 427 |
| Tm | 60.03 | 60.035 | End: 664 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | AAATGCCGCCATGTTTCCAC | AGACTCATCAGCCCCATCCT | Start: 285 |
| Tm | 60.037 | 60.03 | End: 446 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | CAAATGCCGCCATGTTTCCA | AGACTCATCAGCCCCATCCT | Start: 284 |
| Tm | 60.038 | 60.03 | End: 446 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
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| Sequence | AGATGGGGAGCTTTGGGTTG | TCCATTCCCCATCCCCATCT | Start: 7057 |
| Tm | 59.96 | 60.028 | End: 7206 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | ACCAAATCGGCATGGACCAT | CGGCAGGATGTGAACCTCTT | Start: 5746 |
| Tm | 60.033 | 60.036 | End: 6025 |
| Size | 20 | 20 | 280 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATGGGGCTGATGAGTCT | AGCTGCTTCTCCACCCAATC | Start: 427 |
| Tm | 60.03 | 60.035 | End: 664 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | AAATGCCGCCATGTTTCCAC | AGACTCATCAGCCCCATCCT | Start: 285 |
| Tm | 60.037 | 60.03 | End: 446 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | CAAATGCCGCCATGTTTCCA | AGACTCATCAGCCCCATCCT | Start: 284 |
| Tm | 60.038 | 60.03 | End: 446 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | AGATGGGGAGCTTTGGGTTG | TCCATTCCCCATCCCCATCT | Start: 7057 |
| Tm | 59.96 | 60.028 | End: 7206 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
|---|
| Sequence | ACCAAATCGGCATGGACCAT | CGGCAGGATGTGAACCTCTT | Start: 5746 |
| Tm | 60.033 | 60.036 | End: 6025 |
| Size | 20 | 20 | 280 |
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