ssr_primer
Primer Details for TRINITY_DN13982_c5_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTCGCAGCATGAGCATG | TGCATACCAGGCAACGATGT | Start: 5245 |
| Tm | 59.972 | 60.036 | End: 5456 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GCAAACCCCACCATTGTCAC | GAGAAGAACGTCCACAGGCA | Start: 3076 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | GTGCAAACCCCACCATTGTC | GAGAAGAACGTCCACAGGCA | Start: 3074 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | AATGTTGGCCTTTTGCGACC | GCTCGATCCAACTTCCCCAA | Start: 1801 |
| Tm | 59.967 | 60.035 | End: 2007 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | TGCCTGTGGACGTTCTTCTC | CACCCAGAACAGCAGCACTA | Start: 3227 |
| Tm | 59.966 | 59.965 | End: 3454 |
| Size | 20 | 20 | 228 |
(T)31: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTCGCAGCATGAGCATG | TGCATACCAGGCAACGATGT | Start: 5245 |
| Tm | 59.972 | 60.036 | End: 5456 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GCAAACCCCACCATTGTCAC | GAGAAGAACGTCCACAGGCA | Start: 3076 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | GTGCAAACCCCACCATTGTC | GAGAAGAACGTCCACAGGCA | Start: 3074 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | AATGTTGGCCTTTTGCGACC | GCTCGATCCAACTTCCCCAA | Start: 1801 |
| Tm | 59.967 | 60.035 | End: 2007 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | TGCCTGTGGACGTTCTTCTC | CACCCAGAACAGCAGCACTA | Start: 3227 |
| Tm | 59.966 | 59.965 | End: 3454 |
| Size | 20 | 20 | 228 |
(TTA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTCGCAGCATGAGCATG | TGCATACCAGGCAACGATGT | Start: 5245 |
| Tm | 59.972 | 60.036 | End: 5456 |
| Size | 20 | 20 | 212 |
| Primer 2 details |
|---|
| Sequence | GCAAACCCCACCATTGTCAC | GAGAAGAACGTCCACAGGCA | Start: 3076 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | GTGCAAACCCCACCATTGTC | GAGAAGAACGTCCACAGGCA | Start: 3074 |
| Tm | 59.967 | 59.966 | End: 3246 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | AATGTTGGCCTTTTGCGACC | GCTCGATCCAACTTCCCCAA | Start: 1801 |
| Tm | 59.967 | 60.035 | End: 2007 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | TGCCTGTGGACGTTCTTCTC | CACCCAGAACAGCAGCACTA | Start: 3227 |
| Tm | 59.966 | 59.965 | End: 3454 |
| Size | 20 | 20 | 228 |
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