ssr_primer
Primer Details for TRINITY_DN13976_c4_g1_i3 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCCCCTCCCTCCACATA | CATTCCTCCCAGCTCAAGCA | Start: 2035 |
| Tm | 60.03 | 60.035 | End: 2140 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
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| Sequence | CCTTCCCCTCCCTCCACATA | TCCTCCCAGCTCAAGCAAAG | Start: 2035 |
| Tm | 60.03 | 59.963 | End: 2137 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | TTTACGTTGCTCCCCACCTC | ATAAGGCCTCAAAACCGGGG | Start: 3220 |
| Tm | 59.964 | 60.034 | End: 3495 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TAATGGTGAGGCCGATGAGC | ATGTCCTGCTCCAACTTGGG | Start: 845 |
| Tm | 59.893 | 59.961 | End: 971 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TCCATAAGTTGGCCCACACC | TGTGTGTGTCACCTTGGACC | Start: 162 |
| Tm | 59.961 | 60.107 | End: 429 |
| Size | 20 | 20 | 268 |
(TA)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCCCCTCCCTCCACATA | CATTCCTCCCAGCTCAAGCA | Start: 2035 |
| Tm | 60.03 | 60.035 | End: 2140 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCTTCCCCTCCCTCCACATA | TCCTCCCAGCTCAAGCAAAG | Start: 2035 |
| Tm | 60.03 | 59.963 | End: 2137 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | TTTACGTTGCTCCCCACCTC | ATAAGGCCTCAAAACCGGGG | Start: 3220 |
| Tm | 59.964 | 60.034 | End: 3495 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TAATGGTGAGGCCGATGAGC | ATGTCCTGCTCCAACTTGGG | Start: 845 |
| Tm | 59.893 | 59.961 | End: 971 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TCCATAAGTTGGCCCACACC | TGTGTGTGTCACCTTGGACC | Start: 162 |
| Tm | 59.961 | 60.107 | End: 429 |
| Size | 20 | 20 | 268 |
(AC)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCCCCTCCCTCCACATA | CATTCCTCCCAGCTCAAGCA | Start: 2035 |
| Tm | 60.03 | 60.035 | End: 2140 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCTTCCCCTCCCTCCACATA | TCCTCCCAGCTCAAGCAAAG | Start: 2035 |
| Tm | 60.03 | 59.963 | End: 2137 |
| Size | 20 | 20 | 103 |
| Primer 3 details |
|---|
| Sequence | TTTACGTTGCTCCCCACCTC | ATAAGGCCTCAAAACCGGGG | Start: 3220 |
| Tm | 59.964 | 60.034 | End: 3495 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TAATGGTGAGGCCGATGAGC | ATGTCCTGCTCCAACTTGGG | Start: 845 |
| Tm | 59.893 | 59.961 | End: 971 |
| Size | 20 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | TCCATAAGTTGGCCCACACC | TGTGTGTGTCACCTTGGACC | Start: 162 |
| Tm | 59.961 | 60.107 | End: 429 |
| Size | 20 | 20 | 268 |
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