Primer Details for TRINITY_DN1396_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAGAGTATTTTATACCGCACCA | AGGGTGGGTGGATGCATTTT | Start: 241 |
| Tm | 57.28 | 59.883 | End: 391 |
| Size | 24 | 20 | 151 |
| Primer 2 details | |||
| Sequence | CGTACTAGAGTATTTTATACCGCACC | AGGGTGGGTGGATGCATTTT | Start: 238 |
| Tm | 59.201 | 59.883 | End: 391 |
| Size | 26 | 20 | 154 |
| Primer 3 details | |||
| Sequence | ACTAGAGTATTTTATACCGCACCA | CATAGGGTGGGTGGATGCAT | Start: 241 |
| Tm | 57.28 | 59.52 | End: 394 |
| Size | 24 | 20 | 154 |
| Primer 4 details | |||
| Sequence | ACTAGAGTATTTTATACCGCACCAA | AGGGTGGGTGGATGCATTTT | Start: 241 |
| Tm | 57.884 | 59.883 | End: 391 |
| Size | 25 | 20 | 151 |
| Primer 5 details | |||
| Sequence | ACTAGAGTATTTTATACCGCACCA | GGGTGGGTGGATGCATTTTC | Start: 241 |
| Tm | 57.28 | 59.463 | End: 390 |
| Size | 24 | 20 | 150 |
