ssr_primer
Primer Details for TRINITY_DN13963_c0_g1_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACCATACGCGCAATACA | AGTCGTTGGCTTTAACCGCT | Start: 1352 |
| Tm | 59.824 | 60.25 | End: 1616 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | GGACCATACGCGCAATACAC | AGTCGTTGGCTTTAACCGCT | Start: 1353 |
| Tm | 59.42 | 60.25 | End: 1616 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
|---|
| Sequence | AGCGGTTAAAGCCAACGACT | GCATGCATGACACTTACCCC | Start: 1597 |
| Tm | 60.25 | 59.259 | End: 1727 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | AGGGCAAACATGAATGGGTCT | TTCTTTCCCCTAAACGGCCC | Start: 353 |
| Tm | 59.922 | 59.961 | End: 617 |
| Size | 21 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TGGACCATACGCGCAATACA | AGTCGTTGGCTTTAACCGCTA | Start: 1352 |
| Tm | 59.824 | 59.999 | End: 1616 |
| Size | 20 | 21 | 265 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACCATACGCGCAATACA | AGTCGTTGGCTTTAACCGCT | Start: 1352 |
| Tm | 59.824 | 60.25 | End: 1616 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | GGACCATACGCGCAATACAC | AGTCGTTGGCTTTAACCGCT | Start: 1353 |
| Tm | 59.42 | 60.25 | End: 1616 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
|---|
| Sequence | AGCGGTTAAAGCCAACGACT | GCATGCATGACACTTACCCC | Start: 1597 |
| Tm | 60.25 | 59.259 | End: 1727 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | AGGGCAAACATGAATGGGTCT | TTCTTTCCCCTAAACGGCCC | Start: 353 |
| Tm | 59.922 | 59.961 | End: 617 |
| Size | 21 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TGGACCATACGCGCAATACA | AGTCGTTGGCTTTAACCGCTA | Start: 1352 |
| Tm | 59.824 | 59.999 | End: 1616 |
| Size | 20 | 21 | 265 |
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