ssr_primer
Primer Details for TRINITY_DN13954_c0_g5_i1 |
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| (AC)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCCCCGTAAACAACACAGA | GTGCTGCTACTGAACCGGAA | Start: 233 |
| Tm | 59.515 | 60.32 | End: 362 |
| Size | 21 | 20 | 130 |
| Primer 2 details |
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| Sequence | TGTCCCCGTAAACAACACAGA | ATGTGCTGCTACTGAACCGG | Start: 233 |
| Tm | 59.515 | 60.392 | End: 364 |
| Size | 21 | 20 | 132 |
| Primer 3 details |
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| Sequence | TGTCCCCGTAAACAACACAGA | TGCTGCTACTGAACCGGAAA | Start: 233 |
| Tm | 59.515 | 59.605 | End: 361 |
| Size | 21 | 20 | 129 |
| Primer 4 details |
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| Sequence | AGAAAGCCAAAAGGAACGACC | GTGCTGCTACTGAACCGGAA | Start: 103 |
| Tm | 59.046 | 60.32 | End: 362 |
| Size | 21 | 20 | 260 |
| Primer 5 details |
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| Sequence | TGTCCCCGTAAACAACACAG | GTGCTGCTACTGAACCGGAA | Start: 233 |
| Tm | 58.044 | 60.32 | End: 362 |
| Size | 20 | 20 | 130 |
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