ssr_primer
Primer Details for TRINITY_DN13954_c0_g3_i2 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCAAGTCCAAACTGGGGA | GGTGGGGGACTTAGAACTGC | Start: 893 |
| Tm | 59.523 | 60.035 | End: 1140 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
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| Sequence | AGGTCAAGTCCAAACTGGGG | GGTGGGGGACTTAGAACTGC | Start: 892 |
| Tm | 59.521 | 60.035 | End: 1140 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
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| Sequence | TGCCAAAGTTGTGAGGACCT | CATTGGGGGAGCTGGTCAAT | Start: 74 |
| Tm | 59.449 | 60.033 | End: 209 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
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| Sequence | TGCCAAAGTTGTGAGGACCT | GGGGGAGCTGGTCAATTGAA | Start: 74 |
| Tm | 59.449 | 59.96 | End: 205 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
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| Sequence | GTGTCCAAAAGAGGTTGGCC | GAGTTCCATCCCACCTGCAA | Start: 418 |
| Tm | 59.326 | 59.962 | End: 602 |
| Size | 20 | 20 | 185 |
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