ssr_primer
Primer Details for TRINITY_DN13923_c0_g8_i3 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAACGCAGAGCACGTAGC | TGTTTCTTCCCCATTCCCCC | Start: 32 |
| Tm | 60.179 | 59.588 | End: 230 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
|---|
| Sequence | TTTGGAGTAGAGGGTGGGGG | GCACATCTCATTCACCGTGC | Start: 1603 |
| Tm | 60.549 | 59.902 | End: 1812 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | AATTCCATTGCTGTTGGCCG | TGTTTCTTCCCCATTCCCCC | Start: 85 |
| Tm | 59.753 | 59.588 | End: 230 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | CTGTTGGCCGGAAAGACTCA | TGTTTCTTCCCCATTCCCCC | Start: 95 |
| Tm | 60.25 | 59.588 | End: 230 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CACAGGCACAAAACCAACCA | AAGCCTCTGTTTGACTGCCA | Start: 447 |
| Tm | 59.466 | 59.817 | End: 633 |
| Size | 20 | 20 | 187 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAACGCAGAGCACGTAGC | TGTTTCTTCCCCATTCCCCC | Start: 32 |
| Tm | 60.179 | 59.588 | End: 230 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
|---|
| Sequence | TTTGGAGTAGAGGGTGGGGG | GCACATCTCATTCACCGTGC | Start: 1603 |
| Tm | 60.549 | 59.902 | End: 1812 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | AATTCCATTGCTGTTGGCCG | TGTTTCTTCCCCATTCCCCC | Start: 85 |
| Tm | 59.753 | 59.588 | End: 230 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | CTGTTGGCCGGAAAGACTCA | TGTTTCTTCCCCATTCCCCC | Start: 95 |
| Tm | 60.25 | 59.588 | End: 230 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CACAGGCACAAAACCAACCA | AAGCCTCTGTTTGACTGCCA | Start: 447 |
| Tm | 59.466 | 59.817 | End: 633 |
| Size | 20 | 20 | 187 |
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