ssr_primer
Primer Details for TRINITY_DN13915_c3_g3_i5 |
|---|
| (CT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAAGTGTAGCAAGGCGG | AGAAACACCCCACAAAGGCA | Start: 273 |
| Tm | 60.037 | 60.033 | End: 515 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | TGTAGCAAGGCGGGTGAATT | AGAAACACCCCACAAAGGCA | Start: 280 |
| Tm | 59.962 | 60.033 | End: 515 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | CCGCCTTGCTACACTTCAGA | Start: 23 |
| Tm | 59.965 | 60.037 | End: 292 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | AATTCACCCGCCTTGCTACA | Start: 23 |
| Tm | 59.965 | 59.962 | End: 299 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | CCCAAGAATGACGGACCCAA | GTCTTGATGGCACCGAGACA | Start: 1036 |
| Tm | 59.962 | 60.037 | End: 1195 |
| Size | 20 | 20 | 160 |
(TTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAAGTGTAGCAAGGCGG | AGAAACACCCCACAAAGGCA | Start: 273 |
| Tm | 60.037 | 60.033 | End: 515 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | TGTAGCAAGGCGGGTGAATT | AGAAACACCCCACAAAGGCA | Start: 280 |
| Tm | 59.962 | 60.033 | End: 515 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | CCGCCTTGCTACACTTCAGA | Start: 23 |
| Tm | 59.965 | 60.037 | End: 292 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | AATTCACCCGCCTTGCTACA | Start: 23 |
| Tm | 59.965 | 59.962 | End: 299 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | CCCAAGAATGACGGACCCAA | GTCTTGATGGCACCGAGACA | Start: 1036 |
| Tm | 59.962 | 60.037 | End: 1195 |
| Size | 20 | 20 | 160 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAAGTGTAGCAAGGCGG | AGAAACACCCCACAAAGGCA | Start: 273 |
| Tm | 60.037 | 60.033 | End: 515 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | TGTAGCAAGGCGGGTGAATT | AGAAACACCCCACAAAGGCA | Start: 280 |
| Tm | 59.962 | 60.033 | End: 515 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | CCGCCTTGCTACACTTCAGA | Start: 23 |
| Tm | 59.965 | 60.037 | End: 292 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | GTGTCCTCACCCTGTCCTTG | AATTCACCCGCCTTGCTACA | Start: 23 |
| Tm | 59.965 | 59.962 | End: 299 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | CCCAAGAATGACGGACCCAA | GTCTTGATGGCACCGAGACA | Start: 1036 |
| Tm | 59.962 | 60.037 | End: 1195 |
| Size | 20 | 20 | 160 |
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